XB-IMG-133674
Xenbase Image ID: 133674
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Fig. 6. Chordin protein diffuses long-range in Brachet’s cleft of embryos transplanted with Spemann organizer tissue. (A) Diagram of experimental procedure. mGFP was injected in the donor embryo (four injections at the four-cell stage) to trace the lineage of grafted tissue. Secondary axes were complete (with heads, indicated with arrowheads in this photograph) in 75% of the cases (n = 6/8). (B–E) Two embryos transplanted at stage 10 and fixed at stage 12 stained with GFP and Chordin antibodies are shown in optical transverse sections. B and D show localization of the grafted organizer tagged with mGFP; borders are indicated by arrowheads. In C and E, a second gradient of Chordin was observed, with Chordin protein staining in Brachet’s cleft extending a considerable distance from the graft (arrowheads) (n = 19). This indicates that Chordin protein diffuses from the graft through Brachet’s cleft. Image published in: Plouhinec JL et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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