XB-IMG-133675
Xenbase Image ID: 133675
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Fig. S1. Chordin (Chd) depletion increases the BMP signaling (D-V) gradient. (A and B) pSmad1/5/8 immunostainings of injected embryos at stage 12 sectioned transversely between the animal–vegetal axis. pSmad1/5/8 signal is increased in the ventral side in Chd morpholino oligo (MO)-injected embryos (n = 6) compared with wild type (WT) (n = 6). (C) A secreted form of GFP does not diffuse in Brachet’s cleft (arrowhead) after overexpression (n = 5); this indicates that the observed long-distance diffusion of BMP–GFP fusions (zADMP-GFP and xBMP2-GFP) in Brachet’s cleft requires the BMP moiety. ADMP, anti-dorsalizing morphogenetic protein. Image published in: Plouhinec JL et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |