XB-IMG-133743
Xenbase Image ID: 133743
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Fig. 3. Inhibition of serotonin synthesis impairs cilia-driven flow. (A) Experimental setup. Embryos were incubated with TPH inhibitor p-chloro-phenylalanin (PCPA) from stage 25-32, followed by assessment of flow (B), serotonin localization (C) and tph1 expression (D). (B) Reduction of flow velocity in PCPA-treated specimen was partially rescued by co-culture with exogenously applied serotonin. Flow was analyzed by data processing from time-lapse movies following the addition of fluorescent beads to the medium. The mean velocity in control embryo was set to 100%. (C) Absence of serotonin staining (red arrowheads) upon PCPA incubation (Câ²). (D) Unaltered tph1 expression following PCPA treatment (Dâ²). Image published in: Walentek P et al. (2014) Copyright © 2014. Image reproduced with permission of the Publisher.
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