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Xenbase Image ID: 133743

Fig. 3. Inhibition of serotonin synthesis impairs cilia-driven flow. (A) Experimental setup. Embryos were incubated with TPH inhibitor p-chloro-phenylalanin (PCPA) from stage 25-32, followed by assessment of flow (B), serotonin localization (C) and tph1 expression (D). (B) Reduction of flow velocity in PCPA-treated specimen was partially rescued by co-culture with exogenously applied serotonin. Flow was analyzed by data processing from time-lapse movies following the addition of fluorescent beads to the medium. The mean velocity in control embryo was set to 100%. (C) Absence of serotonin staining (red arrowheads) upon PCPA incubation (C′). (D) Unaltered tph1 expression following PCPA treatment (D′).

Image published in: Walentek P et al. (2014)

Copyright © 2014. Image reproduced on Xenbase with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

tph1.L X.laevisThroughout NF stage 32epidermis
secretory epithelial cell
ciliated cell

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