XB-IMG-133744
Xenbase Image ID: 133744
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Fig. 4. The serotonin receptor Htr3 controls cilia motility in the tadpole epidermis. (A,B) Punctate serotonin receptor Htr3 mRNA expression in stage 31 epidermis (A) colocalizes to MCCs, demonstrated by double-staining with anti-acetylated tubulin antibody (B). (C) Reduction of flow velocity in Htr3 morphants. Knockdown of Htr3 by MO1 and MO2 reduced cilia-driven flow velocity compared with control specimens (co). Velocity was partially rescued by co-injection of a mutated Htr3 mRNA not targeted by MO1. Flow was analyzed by adding fluorescent beads to the medium and data processing of time-lapse movies. The mean velocity in control embryo was set to 100%. (D,E) Cilia motility in the epidermis of co-MO and Htr3-MO injected specimens. Cilia were visualized by co-injection of mRFP mRNA. (D) Maximum z-projections (stacks of 450 frames) from time-lapse movies (5-second duration). Blurring indicates motion, acuity lack thereof. (E) Quantification of results. Note rescue of ciliary motility by co-injection of mutated Htr3 mRNA or DNA construct, which is not targeted by Htr3-MO. a, anterior; d, dorsal; l, left; r, right. Image published in: Walentek P et al. (2014) Copyright © 2014. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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