Xenbase Image ID: 133744
Fig. 4. The serotonin receptor Htr3 controls cilia motility in the tadpole epidermis. (A,B) Punctate serotonin receptor Htr3 mRNA expression in stage 31 epidermis (A) colocalizes to MCCs, demonstrated by double-staining with anti-acetylated tubulin antibody (B). (C) Reduction of flow velocity in Htr3 morphants. Knockdown of Htr3 by MO1 and MO2 reduced cilia-driven flow velocity compared with control specimens (co). Velocity was partially rescued by co-injection of a mutated Htr3 mRNA not targeted by MO1. Flow was analyzed by adding fluorescent beads to the medium and data processing of time-lapse movies. The mean velocity in control embryo was set to 100%. (D,E) Cilia motility in the epidermis of co-MO and Htr3-MO injected specimens. Cilia were visualized by co-injection of mRFP mRNA. (D) Maximum z-projections (stacks of 450 frames) from time-lapse movies (5-second duration). Blurring indicates motion, acuity lack thereof. (E) Quantification of results. Note rescue of ciliary motility by co-injection of mutated Htr3 mRNA or DNA construct, which is not targeted by Htr3-MO. a, anterior; d, dorsal; l, left; r, right.
Image published in: Walentek P et al. (2014)
Copyright © 2014. Image reproduced on Xenbase with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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