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Fig. 5. Sall4 knockdown results in a loss of spinal cord differentiation. (A-H) Whole-mount in situ hybridization of (A,C,E,G) uninjected control embryos and (B,D,F,G) embryos injected with 20 ng Sall4 MO into the right A/D blastomere. (A,B) Expression of otx2, krox20 and hoxb9. Arrows indicate the relative anterior-posterior (A-P) position of krox20 and the anterior limit of hoxb9. Dorsal views with anterior to the top. (C-H) Posterior views of hoxb9 (C,D), hoxc10 (E,F) and hoxd10 (G,H) expression. (I-L) Quantification of A-P patterning defects associated with Sall4 knockdown. (I) Distance between the anteriormost expression of otx2 and the first krox20 stripe in arbitrary units (AU). (J-L) Length of the hoxb9 (J), hoxc10 (K) and hoxd10 (L) expression domains (AU). Error bars indicate s.e.m. Means were compared between left and right sides by Student's t-test (*P<0.05, **P<0.01, ***P<0.001). Data were generated from analyzing all embryos shown in A-H. Image published in: Young JJ et al. (2014) Copyright © 2014. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue otx2.L otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 18 optic vesicle eye primordium anterior placodal area neural plate pre-chordal neural plate egr2.L EGR-2, Krox-20, krox20, XKr20, XKrox-20 X. laevis Throughout NF stage 18 presumptive rhombomere hoxb9.L hox 2.5, xhoxb9, XlHbox-6, XlHbox6 X. laevis Throughout NF stage 18 presomitic mesoderm caudal presomitic mesoderm hoxc10.L LOC108709895, MGC80182, XHoxc10 X. laevis Throughout NF stage 18 presomitic mesoderm caudal presomitic mesoderm hoxd10.L hox-4.4, hox4, hox4d, hox4e, LOC116406456, Xhoxd10 X. laevis Throughout NF stage 18 presomitic mesoderm caudal presomitic mesoderm Image source: Published Larger Image Printer Friendly View

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