Xenbase Image ID: 133751

Fig. 6. Knockdown of Sall4 causes an increase in pou5f3 expression. (A-I) Whole-mount in situ hybridization of (A,D,G) uninjected control embryos, (B,E,H) embryos injected with 20 ng Sall4 MO into the right A/D blastomere, and (C,F,I) embryos injected bilaterally with 40 ng Sall4 MO (20 ng/blastomere at the 2-cell stage), showing expression of (A-C) pou5f3.2, (D-F) pou5f3.3 and (G-I) pou5f3.1. Dorsal views, anterior to the top. (J-L) qPCR for pou5f3.2 (J), pou5f3.3 (K) or pou5f3.1 (L) in uninjected embryos, embryos injected with 40 ng Sall4 MO (20 ng/blastomere at the 2-cell stage), and embryos injected with 40 ng Sall4 MO (20 ng/blastomere at the 2-cell stage) plus 500 pg X. tropicalis sall4 RNA (250 pg/animal-dorsal blastomere at the 4-cell stage). The expression is relative to odc. Error bars indicate s.e.m. Means compared with uninjected control by one-way ANOVA followed by Tukey post-hoc analyses (*P<0.05). n=4 experiments. Image published in: Young JJ et al. (2014) Copyright © 2014. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue pou5f3.L Oct-91, oct4, oct79, oct91, oct92, pou5f3.1, XLPOU91, xoct-91, xoct91 X. laevis Sometime during NF stage 15 to NF stage 16 neural plate pre-chordal neural plate chordal neural plate pou5f3.2.L Oct-25, oct25, pou25, xoct-25, xoct25 X. laevis Sometime during NF stage 15 to NF stage 16 mesoderm rostral presomitic mesoderm neural plate chordal neural plate pou5f3.3.L oct-60, oct60, pou60, XLPOU-60, XLPOU60, xoct60 X. laevis Sometime during NF stage 15 to NF stage 16 neural plate pre-chordal neural plate chordal neural plate Image source: Published Larger Image Printer Friendly View

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