Xenbase Image ID: 133753

Fig. 8. Loss of spinal cord in Sall4 morphants is due to an increase in pou5f3 expression. (A-L) Whole-mount in situ hybridization of (A-D) uninjected control embryos, (E-H) embryos injected with 40 ng Sall4 MO (20 ng/blastomere at the 2-cell stage), or (I-L) embryos injected with 40 ng Sall4 MO, 20 ng Pou5f3.2 MO, 10 ng Pou5f3.3a MO, 10 ng Pou5f3.3b MO, and 20 ng Pou5f3.1 MO. (N) Embryos injected with 20 ng Pou5f3.2 MO, 10 ng Pou5f3.3a MO, 10 ng Pou5f3.3b MO and 20 ng Pou5f3.1 MO. Expression is shown for (A,E,I) otx2, krox20 and hoxb9, (B,F,J) hoxc10, (C,G,K) hoxd10 and (D,H,L,N) sox2. Dorsal views with anterior to the top. (M) Quantification of posterior neural gene expression as measured by expression domain length in arbitrary units (AU). White, uninjected control embryos. Gray, embryos injected with 40 ng Sall4 MO (20 ng/blastomere at the 2-cell stage). Black, embryos injected with 40 ng Sall4 MO, 20 ng Pou5f3.2 MO, 10 ng Pou5f3.3a MO, 10 ng Pou5f3.3b MO and 20 ng Pou5f3.1 MO. Error bars indicate s.e.m. Means compared with uninjected control by one-way ANOVA followed by Tukey post-hoc analyses (***P<0.001). Data were generated from analyzing all embryos shown in A-C, E-G, and I-K. Image published in: Young JJ et al. (2014) Copyright © 2014. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue otx2.L otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 18 optic vesicle eye primordium neural plate pre-chordal neural plate anterior placodal area egr2.L EGR-2, Krox-20, krox20, XKr20, XKrox-20 X. laevis Throughout NF stage 18 presumptive rhombomere hoxb9.L hox 2.5, xhoxb9, XlHbox-6, XlHbox6 X. laevis Throughout NF stage 18 presomitic mesoderm caudal presomitic mesoderm hoxc10.L LOC108709895, MGC80182, XHoxc10 X. laevis Throughout NF stage 18 presomitic mesoderm caudal presomitic mesoderm hoxd10.L hox-4.4, hox4, hox4d, hox4e, LOC116406456, Xhoxd10 X. laevis Throughout NF stage 18 presomitic mesoderm caudal presomitic mesoderm sox2.S anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Throughout NF stage 18 neural plate pre-chordal neural plate chordal neural plate neural tube anterior neural tube posterior neural tube Image source: Published Larger Image Printer Friendly View

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