XB-IMG-133852
Xenbase Image ID: 133852
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Fig. 3. Xenopus laevis basic transcription element binding
protein (BTEB) activates transcription from promoters containing
single or multiple GC boxes. CV1 cells were transfected as
described in the Materials and Methods and cultured for 24 h
before harvesting for analysis of chloramphenicol acetyl
transferase (CAT) expression. CAT content was normalized to
total cellular protein, and the data are expressed as fold change
relative to the control (empty expression vector transfected
cells = 1). (a) CV-1 cells were co-transfected with the designated
expression vectors plus the pSV2CAT promoter/reporter
plasmid (this native promoter contains six contiguous GC box
sequences.) The data in the graph represent the mean (± SEM)
of three separate transfection experiments. (b) CV-1 cells were
co-transfected with the designated expression vectors and
promoter/reporter vectors. The pSV/MC53 vector drives CAT
expression and contains the SV40 enhancer and a single GC
box sequence. The data in the graph represent the mean
(± SEM) of three replicates in one transfection experiment.
Asterisks in both upper and lower panels designate significant
differences from the controls (*, P < 0.05; **, P < 0.01 by
Student’s unpaired t-test). Image published in: Hoopfer ED et al. (2002) Copyright © 2002. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View |