XB-IMG-133853
Xenbase Image ID: 133853
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Fig. 4. Dominant negative activity of xBTEB[DBD] on the xTR
promoter in Xenopus laevis XTC-2 cells. XTC-2 cells were transfected
with 1 μg xTR -promoter-CAT and varying amounts of
pCMV-xBTEB[DBD] as described in the Materials and Methods.
Cell extracts were analyzed for (a) basic transcription element
(BTE)-binding activity by electrophoretic mobility shift assay
(EMSA; P, probe only) or (b) for chloramphenicol acetyl transferase
(CAT) expression by CAT ELISA. In (b), cells were treated
with or without 30 nM 3,5,3 -triiodothyronine (T3) for 24 h before
harvest. Means of three replicates with SEM are given. Image published in: Hoopfer ED et al. (2002) Copyright © 2002. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View |