XB-IMG-133855
Xenbase Image ID: 133855
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Fig. 6. 3,5,3 -triiodothyronine (T3)
upregulates Xenopus basic transcription
element binding protein
(xBTEB) mRNA in the brain
(diencephalon), intestine and tail of
premetamorphic Xenopus laevis
tadpoles. Stage 52 X. laevis
tadpoles were treated with either
50 nM (for brain and intestine blots)
or 200 nM T3 (for tail blot) in the
aquarium water and tissues were
collected at various times thereafter
and analyzed by northern
blot. Representative northern blots
of xBTEB mRNA expression in the
(a) diencephalon, (b) intestine and
(c) tail, respectively. Graphs show
the densitometric quantitation of
northern blot data (n = 3 per
tissue). Bars in the graphs represent
the mean mRNA level, expressed
as a percentage of maximal
expression (n = 3 blots per tissue).
(d) xBTEB gene expression during
metamorphosis depends on
thyroid hormone. Northern blot of
xBTEB mRNA expression in the
diencephalon of prometamorphic
(Nieuwkoop and Faber stage 58)
X. laevis tadpoles treated with the
goitrogen methimazole (1 mM) for
7 days. Image published in: Hoopfer ED et al. (2002) Copyright © 2002. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View |