XB-IMG-133857
Xenbase Image ID: 133857
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Fig. 8. Xenopus basic transcription element binding protein (xBTEB) in the tadpole brain is developmentally and hormonally regulated and is not expressed in proliferating cells. Double labeling immunohistochemistry was used to analyze xBTEB protein and bromodeoxyuridine (BrdU)-labeled cells.
Premetamorphic tadpoles (Nieuwkoop and Faber [NF] stage
52; panels A–F) were reared without (A–C) or with (D–F)
50 nM T3 for 48 h. BrdU was added to the aquarium water to
a final concentration of 500 μM 3 h before tadpoles were killed,
to label mitotic cells. Saggital sections (10 μm) were analyzed
by double labeling immunohistochemistry for xBTEB (red)
and BrdU (green; the optic tectum and cerebellum are
shown). Note that xBTEB protein is not expressed in proliferating
cells identified by BrdU labeling. Late prometamorphic/
early climax stage tadpoles (NF stage 60) were reared in the
(G) absence or (H) presence of 50 nM T3 for 48 h before they
were killed. The panels show xBTEB immunoreactivity in the
optic tectum. Bars, 100 μm. Image published in: Hoopfer ED et al. (2002) Copyright © 2002. Image reproduced with permission of the Publisher, John Wiley & Sons.
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