XB-IMG-133860
Xenbase Image ID: 133860
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FIGURE 3.
A, binding of GST-xBTEB1[DBD] to regions of the proximal X. laevis TrβA promoter in vitro. We used EMSA to test the ability of radioinert DNA fragments (1.89 μM/reaction) corresponding to different regions of the proximal TrβA promoter (generated by PCR; see Fig. 2 and supplemental Table 1) to displace GST-xBTEB1[DBD] binding to the 32P-BTE probe. mBTE, mutated BTE. B, binding of GST-xBTEB1[DBD] to GC-rich regions of the proximal TrβA promoter. We used EMSA to test whether GST-xBTEB1[DBD] could bind to short 32P-labeled oligonucleotides encompassing one or two GC boxes in the TrβA promoter. The numbering of the GC boxes included in each oligonucleotide probe is based on that given in Fig. 2 and Table 4. In each case homologous, radioinert competitors (1.89 μM) were used to displace binding. Image published in: Bagamasbad P et al. (2008) Copyright © 2008. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |