Xenbase Image ID: 133861

FIGURE 4. BTEB1 associates with the proximal TrβA promoter in vivo in a T3 and developmental stage-dependent manner. ChIP assay was conducted using an affinity-purified IgG directed against the N-terminal region of X. laevis BTEB1. A, T3-dependent association of BTEB1 with the proximal TrβA promoter in tadpole brain and tail. Premetamorphic (NF stage 52) X. laevis tadpoles were treated with 10 nM T3 added to the aquarium water for 48 h prior to tissue collection for ChIP assay (see “Experimental Procedures”). The lettered TrβA promoter regions analyzed correspond to those given in Fig. 2 and supplemental Table 1. The TrβA exon 3/exon 4, and the Efα1 and Ifabp promoters were used as negative controls. B, developmental stage-dependent association of BTEB1 with the proximal TrβA promoter in early prometamorphic (NF stage 54) and climax stage (NF stage 62) X. laevis tadpole brain. Only region A of TrβA promoter, which showed robust T3-dependent association of BTEB1 was targeted for ChIP analysis in this experiment. Each of the ChIP experiments was repeated three times with similar results. Image published in: Bagamasbad P et al. (2008) Copyright © 2008. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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