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FIGURE 6. Expression of BTEB1 enhances TrβA autoinduction in XTC-2 cells. A, forced expression of BTEB1 accelerates and enhances autoinduction of the TrβA promoter. XTC-2 cells were cotransfected with the X. laevis TrβA promoter-luciferase plasmid, pCMV-xBTEB1, and pRenilla (to normalize for transfection efficiency using a dual reporter luciferase assay; see “Experimental Procedures”). Cells were treated with 5 nM T3 for 0, 2, or 6 h before harvest. Bars represent the mean ± S.E. The data shown are the means ± S.E. from one transfection experiment (n = 4/treatment group), and the experiment was repeated three times with similar results. The T3-dependent activation of the TrβA promoter was not altered by transfection with empty vector (pCMVneo; data not shown.) Asterisks denote significant differences from empty vector controls (*, p < 0.01; **, p < 0.001; Student's unpaired t test). B, forced expression of BTEB1 increases the expression of endogenous TrβA mRNA. XTC-2 cells were cotransfected with 1 μg of pCS2-xBTEB1 or pCS2 empty vector. Forty eight h after transfection the cells were treated with 5 nM T3 for 0, 2, 4, or 6 h before harvest. Gene expression analysis was done by RTqPCR. Data shown are the means ± S.E. from one transfection experiment (n = 6/treatment), and the experiment was repeated four times with similar results. Asterisks denote significant differences from empty vector controls (*, p < 0.05; **, p < 0.001; unpaired t test). Image published in: Bagamasbad P et al. (2008) Copyright © 2008. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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