Xenbase Image ID: 133935

Figure 7. FoxA4 inhibition affected anterior ectodermal/neural specification. Embryos at early neural plate stage injected with 20 ng of CtrlMO or of FoxA4MO before the first cleavage (A, B, F, G, K–N, P–S) or unilaterally injected into 1 cell at the 2-cell stage (C, D, H, I, O, T). The injected side was determined by DOG fluorescence (insets). Embryos were hybridised with the following probes: Xanf1, en2, and hoxb7 (A–C, F–H); hoxc6 (D, I); otx2 and krox20 (K, L, P, Q); Xag1 and N-tubulin (M, N, R, S); en2 and hoxb1 (O, T). Green arrows in (A, F) point to the anterior limit of hoxb7. At neural plate stage, Xag1 marks the cement gland anlage (indicated between red arrowheads in N), and the hatching gland primordia (red arrows in N) [98] [90]. Black arrowhead in (H), caudal shift of Xanf1 and fusion with the en2 stripe. Black arrow in (H), fusion of the SHA and the ANR. Red arrow in (I), down-regulation of hoxc6 on the injected side. Yellow arrowhead in (Q), diffuse CGA and anterior border of the ANR. Notice the down-regulation and the caudal shift of en2 (red arrows) and hoxb1 (green arrows) by comparing the injected side (right) with the non-injected side (left) in the FoxA4 morphant in (T) and with the CtrlMO-injected embryo in (O). (E) Ratio between the distance from the posterior limit of Xanf1 to the blastopore and the total length of the embryo in the groups shown in A, B, F, G (r-Xanf1). The ratio was significantly lower in FoxA4 morphants; P<0.0001, two-tailed t-test. (J) The ratio between the length of the hoxb7 domain and the embryo’s length was significantly lower in FoxA4 morphants, as measured in the groups shown in A, B, F, G; P<0.0001, two-tailed t-test. ANR, anterior neural ridge; CGA, cement gland anlage; SHA, stomodeal-adenohypophyseal anlage; m/h, midbrain/hindbrain boundary; m, d, presumptive mesencephalic and caudal diencephalic regions expressing otx2; r3, third rhombomere; r4, fourth rhombomere. (A, D, F, I, K, M, O, P, Q, R, T) are dorsal views; (B, C, G, H, L, N, Q, S) are anterior views. doi:10.1371/journal.pone.0110559.g007 Image published in: Murgan S et al. (2014) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue en2.L En-2, en2-a, en2-b, eng2, engrailed 2, engrailed homolog 2, engrailed-2, maben, xen2 X. laevis Throughout NF stage 19 presumptive midbrain-hindbrain boundary hesx1.L anf2, hesx1-a, hesx1-b, rpx, Xanf, Xanf-1, XANF-2, Xanf1, Xanf2 X. laevis Throughout NF stage 19 anterior placodal area anterior neural ridge adenohypophyseal placode hoxc6.L ACI, xeb1, XlHbox1 X. laevis Throughout NF stage 19 presomitic mesoderm caudal presomitic mesoderm egr2.L EGR-2, Krox-20, krox20, XKr20, XKrox-20 X. laevis Throughout NF stage 19 presumptive rhombomere otx2.L otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 19 anterior placodal area optic vesicle adenohypophyseal placode cement gland primordium ag1.L ag, ag1-a, ag1-b, XAG, xag-1, xag1, Xag2 X. laevis Throughout NF stage 19 anterior placodal area cement gland primordium tubb2b.L N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 19 neuroectoderm neuron Rohon-Beard neuron neural progenitor cell hoxb1.L hox-2.9, hoxb-1, XHox-b1 X. laevis Throughout NF stage 19 presomitic mesoderm rostral presomitic mesoderm caudal presomitic mesoderm presumptive rhombomere Image source: Published Larger Image Printer Friendly View

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