Xenbase Image ID: 133959

Fig. 1. Dkk1 acts at the top of the program for the myeloid cell differentiation at the ventral blood island (aVBI). (a–l) Whole-mount in situ hybridization analysis was performed to show the expression of c/ebpa (a, b), spib (c–g) and mpo (h–l) in ventral marginal zone (VMZ) explants that had been injected with designated mRNAs or in VMZ explants without injection. RNA was injected into the prospective VMZ at the 4-cell stage, and VMZ tissues excised from the embryo were cultured until st. 18, st. 25 or st. 30. Injection of dkk1 mRNA (50 pg) induced the expression of c/ebpa, spib and mpo (b, d, i). Overexpression of c/ebpa also induced a certain level of spib expression (e, f, g) and mpo (j, k, l), but intensity of expression was lower than that in the explants injected with dkk1 mRNA. (m) Reverse transcriptionpolymerase chain reaction (RT-PCR) analysis was done to show the expression of mpo in whole embryos (WE) at st. 25 (lane 1), WE without RT reaction (lane 2), in VMZ explants without injection (lane 3), in VMZ explants injected with dkk1 (lane 4), in VMZ explants injected with increasing doses of c/ebpa (lanes 5–7). Image published in: Sakata H and Maéno M (2014) Copyright © 2014. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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