XB-IMG-133962
Xenbase Image ID: 133962
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Fig. 4. Effect of Nkx2.5 morpholino oligos (MO) in the myeloid cell lineage is partially recovered by simultaneous injection of c/ebpa
mRNA. (a–k) Designated mRNAs (dkk1, 50 pg; c/ebpa, 1 ng; spib, 300 pg) and Nkx2.5 MO (18.4 pmol) were injected into the ventral
marginal zone (VMZ) at the 4-cell stage. The VMZ explants were cultured until the appropriate stages and processed for whole-mount in
situ hybridization analysis to examine the expression of c/ebpa (a–c), spib (d–g), and mpo (h–k). Expression of myeloid markers was
induced by dkk1 mRNA injection (b, e, i), and expression of spib and mpo was suppressed by injection of Nkx2.5 MO (f, j). The expression
of spib and mpo was then restored by injection of c/ebpa mRNA (g, k). The number in each panel indicates total number of samples
from three independent experiments. (l) RT-PCR analysis was done to show the expression of c/ebpa, spib, mpo and ef1a in whole
embryos (WE) at st. 25 (lane 1), in WE without RT reaction (lane 2), in VMZ explants without injection (lane 3), in VMZ explants injected
with 50 pg dkk1 (lane 4), with 50 pg dkk1 and 18.4 pmol Nkx2.5 MO (lane 5), and with 50 pg dkk1, 18.4 pmol Nkx2.5 MO, and 1 ng
c/ebpa (lane 6). Image published in: Sakata H and Maéno M (2014) Copyright © 2014. Image reproduced with permission of the Publisher, John Wiley & Sons.
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