Xenbase Image ID: 134184

Fig. 3. Spatial expression of Xprx- 1 at stage 9. RT-PCR and Southern blot analysis showing localized expressions of Xprx-1. Fifty embryos were dissected into an animal cap (AC). vegetal cap (VC), dorsal marginal zone (DMZ), lateral marginal zone (LMZ). and ventral marginal zone (VMZ), as shown in the diagram at the right. Total RNA was isolated and used for RT-PCR and Southern blot analysis. EF1-a is an VC internal loading control that is expressed ubiquitously in embryos (Krieg et at. 1989). WE (whole embryo, stage 9) is a positive control, and RT- (reverse transcriptase was not added to the total WE RNA) is a negative control. Image published in: Takahashi S et al. (1998) Copyright © 1998. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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