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Fig. 5. Expression of Xprx-1 in animal caps treated with peptide growth factors. Total RNA was isolated from animal caps treated with different concentrations of Activin A or bFGF for 6 h. RT-PCR and Southern blot analysis showed that Activin A dose dependently induced expression of Xprx-1 genes, but that bFGF did not. EF1-a served as an internal loading control. WE (whole embryo, stage 20) was a positive control, and RT- (reverse transcriptase not added to total WE RNA) was a negative control.

Image published in: Takahashi S et al. (1998)

Copyright © 1998. Image reproduced with permission of the Publisher, John Wiley & Sons.

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