XB-IMG-134187
Xenbase Image ID: 134187
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Fig. 6. Temporal expression of Xprx-1 in animal caps treated
with Activin A. Animal caps were treated in Steinberg's solution
with or without 100 ng/ml Activin A for 30 min. After treatment,
the animal caps were maintained in normal Steinberg's solution
for various periods (0-24 h). RT-PCR and Southern blot analysis
showed induction of zygotic Xprx-1 expression after 6 h in
Activin A-treated animal caps, but not in non-treated animal caps.
EF1-a served as an internal loading control. WE (whole embryo,
stage 20) is a positive control, and RT- (reverse transcriptase not
added to total WE RNA) is a negative control. Image published in: Takahashi S et al. (1998) Copyright © 1998. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View |