XB-IMG-134310
Xenbase Image ID: 134310
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FIG. 2. Keller explants express neural and mesodermal markers with correct anterior–posterior patterning. (A–H) Neural markers (green
arrows: Krox-20 for hindbrain; HoxB9 for spinal cord; nrp-1 for panneural; Xotx-2 for forebrain) and cement gland marker (red arrows,
XAG-1) in explants (A–D) and whole embryos (E–H). All explants and embryos stained at stage 22 except for D and H which were stained
at stage 15. bp, blastopore. (D) Note both mesodermal (m) and neural expression (n) of Xotx-2 and location of glass bead (gb). Occasionally,
in situ hybridization resulted in a brown-staining product (D,H) but the reason for this variation is not understood. (I,J) Expression of early
mesodermal markers in stage 10-minus explants (I, red arrows) and stage 10.5 gastrulae (J). I. Anterior mesoderm (gsc) occupies the lower
edge of the explant, while more posterior markers (Xbra, Xnot) are found in the interior. Dots in J indicate dorsal lip. Image published in: Holowacz T and Sokol S (1999) Copyright © 1999. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |