Xenbase Image ID: 134312

FIG. 4. A dominant-negative FGF receptor (XFD) inhibits posterior neural development in explants. (A) RNA injection in the dorsal midline at the two-cell stage (4 injection sites, total of 4 ng RNA per embryo). (B) Control d50-injected embryo at the tailbud stage. Black arrow, En-2. Red arrow, XAG-1. (C) XFD-injected embryo expresses XAG-1 and En-2. Note lack of blastopore closure. (D) Control (d50 RNA) explants express cement gland and En-2. (E) Explants isolated from XFD embryos lack En-2. Cement gland develops normally. Explants in F and G were obtained from embryos injected with RNA in the dorsal midline and lateral regions. XFD-expressing explants are marked by an asterisk. Black arrows indicate glass bead that was placed in the center of the explant. (F) Control explants express XAG-1 (white arrow) and HoxB9 (green arrows). XFD explants (*) do not express HoxB9, although they do have cement gland. (G) Xotx-2 (white arrows) is expressed in explants. All explants were stained at stage 22, except ones in G, which were stained at stage 15. Image published in: Holowacz T and Sokol S (1999) Copyright © 1999. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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