XB-IMG-134314
Xenbase Image ID: 134314
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FIG. 6. Posterior neural markers are lost when XFD is expressed throughout the marginal zone. (A) XFD RNA (lanes 1 and 3) and d50 RNA
(lanes 2 and 4) were injected at the two-cell stage into either the dorsal regions (“D,” lanes 1 and 2; 4 injection sites, total of 6 ng of RNA
per embryo) or into the dorsal and lateral regions (“D 1 L,” lanes 3 and 4; 6 injection sites, total of 8–10 ng of RNA per embryo). cDNA
for RT–PCR was prepared from stage 27 embryos. Although injection of XFD in dorsal regions inhibits muscle actin expression (m-actin,
lane 1), loss of posterior neural markers (En-2; Krox-20, HoxB9) is only seen when injection is targeted to both the dorsal and lateral regions
(lane 3). EF-1a is a loading control. (B) RT–PCR analysis of embryos (lanes 6 and 7) injected with RNA as in lanes 3 and 4. Dorsal marginal
zone (DMZ) explants were prepared from stage 10-minus gastrulae and cultured until stage 27 (lanes 8 and 9). Note loss of posterior neural
markers and up-regulation of XAG-1 and Xotx-2 due to XFD RNA overexpression (lane 8). EF-1a is a loading control. (C,D) Dorsal marginal
zone explants isolated from embryos that had been injected with control d50 RNA (C) or XFD RNA (D) and stained for En-2 (dark blue) and
XAG-1 (light blue). Image published in: Holowacz T and Sokol S (1999) Copyright © 1999. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |