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FIG. 2. DRAGON expression enhances BMP signaling and sensitizes cells to BMP. A, LLC-PK1 renal epithelial cells were transiently transfected with BRE-Luciferase construct (BRE-Luc) alone or in combination with 2 or 20 ng of a DRAGON expression construct. Luciferase activity was assessed after incubation in the absence (white bars) or presence (black bars) of BMP2 (1 nM) in the culture medium. Luciferase activity is represented as relative light units (RLU). DRAGON increases and potentiates BMP2-mediated signaling. B, DRAGON-mediated BMP signaling is ligand-dependent. LLC-PK1 cells were transiently transfected with BRE-Luc in the presence or absence of DRAGON and treated with increasing amounts of Noggin as indicated. As a positive control, BRE-Luc-transfected LLC-PK1 cells were treated with BMP2 (1 nM) in the presence of Noggin (50 ng/ml). As a negative control, LLC-PK1 cells transfected with the TGF -responsive reporter construct MLP-Luc were treated with TGF (1 nM) in the presence of Noggin (50 ng/ml). C, 10 T1/2 cells transiently transfected with the BMP-responsive I-BRE-Luc reporter and DRAGON were incubated with increasing doses of BMP2 (25–500 pM). Image published in: Samad TA et al. (2005) Copyright © 2005. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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