Xenbase Image ID: 134352

FIG. 6. DRAGON potentiates BMP signaling in Xenopus embryos. A, microinjection of mouse DRAGON mRNA in Xenopus laevis embryos at gastrula stages synergizes with Smad1 (mRNA injection) to induce mesodermal (xBra and mix1) and endodermal (mixer) markers, as assessed by reverse transcription (RT)- PCR. No induction of EK or Sox2 occurs. B, in animal cap explants DRAGON mRNA injection induces expression of the anterior neural markers nrp1 and Otx2, as well the as cement gland marker (Xag) and nkx2.5, a heart marker. Orthidine decarboxylase (ODC) is the loading control. C, Xenopus embryos were injected in the animal pole of one of two cells at the two-cell stage with DRAGON mRNA and analyzed at late neurula (stage 23), for changes in neural crest patterning, and early tadpole stages (stage 28) for ectopic induction of neural tissue. DRAGON overexpression inhibits twist RNA, a neural crest marker (top panels), and induces ectopic patches of N-tubulin, a neural marker (lower panels). Image published in: Samad TA et al. (2005) Copyright © 2005. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue twist1.S twist, twist1-a, twist1-b, X-twi, Xtwi, Xtwist X. laevis Throughout NF stage 23 neural crest cranial neural crest mandibular crest hyoid crest branchial crest tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 28 central nervous system brain spinal cord Image source: Published Larger Image Printer Friendly View

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