Xenbase Image ID: 134373

Fig. 5. (A) Interactions of the microdomain (MD) of egg membrane with the HPX peptide (473–485; GMSQIRGETFFFK), showing binding of the MD to the HPX peptide captured on a sensor chip for SPR system (Biacore). Binding of the MD was inhibited in the presence of GM1, but not of asialo GM or GM3. RU, resonance unit (1 pg/mm2). (B) The presence of uroplakin III (UPIII) in the MD bound to the HPX peptide was confirmed by further binding of anti-UPIII antibody on the chip. (C–H) Detection of GM1 on the sperm surface with FITC-conjugated cholera toxin B subunit (FITC-CTB), showing a very small amount of GM1 on the GM1-untreated sperm (C) in comparison with the sperm without treatment of FITC-CTB (D), but a large amount of GM1 bound the sperm after treatment of the sperm with GM1 (100 µg/mL, 15 min) (E and F). The binding of GM1 on the sperm was inhibited by the pre-treatment of sperm with anti-MMP-2 HPX antibody (1:10-dilution, 20 min) (G and H). The set of E and F or G and H is showing different optical slices in the same sperm, respectively. Left panels, the fluorescence images of FITC-CTB; Right panels, the merge of fluorescence images with differential interference images (DIC). Ac, acrosomal region; H, head; T, tail. Bar, 5 µm. Image published in: Iwao Y et al. (2014) Copyright © 2014. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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