XB-IMG-134375
Xenbase Image ID: 134375
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Fig. 7.
Changes of voltage-dependency in fertilization by the treatment of sperm with gangliosides or anti-MMP-2 HPX antibody. (A–D) Jellied eggs were voltage-clamped at +10 mV and then inseminated. At 10 min after insemination, the clamp voltage was negatively shifted in incremental steps (10 mV-step, for 5 min). Fertilization current (egg activation) was elicited at –20 mV by untreated sperm (A), but at 0 mV by the sperm which had been treated with GM1 (100 µg/mL, 15 min) (B), or by the sperm which had been treated with anti-MMP-2 HPX antibody (1:100-dilution, 15 min) (C). Untreated sperm caused activation in about half of the eggs at –10 mV or –20 mV (untreated), but 10% and 80% of the eggs were activated by GM1-treated sperm at 0 mV and –10 mV, respectively (GM1). The treatment of sperm with asialo GM1 or GM3 (100 µg/mL, 15 min) did not affect the voltage dependency (asialo GM1, GM3). The sperm which had been treated with anti-MMP-2 HPX antibody (1:100-dilution, 15 min) caused activation in 20% and 60% of the eggs at 0 mV and −10 mV, respectively (anti-MMP-2 HPX). No egg was activated at 0 mV by the sperm treated by preimmune antibody (preimmune). Each column represents results from 10 to 30 eggs. Image published in: Iwao Y et al. (2014) Copyright © 2014. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |