Xenbase Image ID: 134377

Fig. S1. (A) Amino acid sequence and domain structure of Xenopus laevis testis MMP-2, showing a signal peptide (Pre), a prodomain (Pro), a typical catalytic domain (Cat) with zinc binding region (underline), a fibronectin-like collagen-binging domain (FN), and a hemopexin domain (HPX). Asterisk, glutamic acid (E) in Xenopus laevis tadpole tail MMP-2 (GenBank, AY037943). Arrows, the position of primers for RT-PCR. Dotted line, HPX peptide. (B) RT-PCR analysis for confirmation of the expression of MMP-2 in Xenopus laevis testis. RT reaction was performed with GeneRacer kit (Invitrogen), and poly (A) RNA extract was used as a negative control (RT−). The reaction products were subjected to PCR (95 °C for 30 sec, 56 °C for 1 min, 72 °C for 2 min) for 30 cycles; the forward and reverse PCR primers specific for MMP-2 were 5′-CTGCACTGATTCTGGTCGCTC-3′ and 5′-TCAACACGATCAACATCAGGA-3′, respectively. Image published in: Iwao Y et al. (2014) Copyright © 2014. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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