XB-IMG-134392
Xenbase Image ID: 134392
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FIG. 1. Whole-mount TUNEL staining reveals dying cells in gastrulation-stage embryos. (A) Stage 10.5, vegetal view, no TUNEL staining
was detected in this embryo. (B) Stage 10.5, lateral view, a small patch of TUNEL staining is evident in the equatorial region of the embryo.
(C) Stage 10.5, vegetal view, hundreds of TUNEL-positive nuclei were evident in the circumblastoporal region. (D) Stage 10.5, lateral view,
TUNEL-stained nuclei covered the surface of the embryo except for the blastopore. (E) Stage 11.5, vegetal view, a patch of TUNEL staining
in the equatorial region extends to the edge of the blastopore. (F) Stage 11.5, lateral view, two bilaterally symmetrical patches of TUNEL
staining border the blastopore. (G) Stage 11.5, animal view, TUNEL staining was detected in the animal pole and extended vegetally to the
edge of the blastopore. (H) Stage 11.5, lateral view, a partially exogastrulating embryo showing an accumulation of TUNEL-positive nuclei
in the blastopore (arrowhead). (I) Stage 12.5, animal view (left), lateral view (right). A dorsal median stripe of TUNEL-stained nuclei
extended from the blastopore. (J) Stage 12.5, animal view (left), lateral view (right). TUNEL-stained nuclei were detected across the dorsal
side of the embryo. Throughout gastrulation TUNEL staining was never detected in the blastopore, indicated by the dotted red line. The
embryos shown are representative of the TUNEL staining observed following analysis of 237 embryos of which 67% were stained. Embryos
with more than 5 TUNEL-stained nuclei were considered TUNEL positive. Image published in: Hensey C and Gautier J (1998) Copyright © 1998. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |