Xenbase Image ID: 134395

FIG. 4. Programmed cell death as detected by whole-mount TUNEL staining at the late neural stage. Each panel shows a lateral (top), and a dorsal (bottom) view. (A) Stage 18, TUNEL staining in the anteriormost portion of the developing brain. A clear asymmetry is present. (B) Stage 18, TUNEL staining in the posterior of the embryo, along the neural fold. (C) Stage 18, bilaterally symmetrical staining in the anterior developing brain, and faint staining in the posterior of the embryo. (D) Stage 23, bilaterally symmetrical diffuse staining in the developing head possibly corresponding to migrating neural crest cells. (E) Stage 23, diffuse staining in the developing head, showing a distinct right–left asymmetry. (F) Stage 25, diffuse TUNEL staining throughout the head and tailbud. The staining shown is representative of the programmed cell death seen following staining of 89 embryos of which 98% were TUNEL positive. Embryos with more than 5 TUNEL-stained nuclei were considered TUNEL positive. Image published in: Hensey C and Gautier J (1998) Copyright © 1998. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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