Xenbase Image ID: 134397

FIG. 6. PCD as detected by whole-mount TUNEL staining during stages 29–35. Each panel shows a lateral (top), and dorsal view (bottom). (A) Bilaterally symmetrical TUNEL staining is detected in the fore- and midbrain; lighter staining is detected in the eye vesicle. Line indicates the approximate location of a sagital section shown in Fig. 7F. (B) TUNEL staining is detected in the brain, eye vesicles, the posterior half of the spinal cord, and the elongating tail. (C) Dying cells were detected in the brain and developing eye. (D) TUNEL staining in the rhombencephalon, the outer ring of the eye vesicle the branchial arches (arrowheads), and the otic vesicle. Staining was also evident along the length of the spinal cord and in the tail tip. The staining is representative of the patterns seen following staining of 92 embryos of which 99% were TUNEL stained. Embryos with more than 5 TUNEL-stained nuclei were considered TUNEL positive. Image published in: Hensey C and Gautier J (1998) Copyright © 1998. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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