Xenbase Image ID: 134455

Fig.2. Glut1 immunolocalization in amphibian retina. (A) Western blot analysis of retinal extracts from mouse (lane 1) and Xenopus (lane 2), probed with anti-human Glut1 polyclonal antibody. The Xenopus isoform was not recognized. (B) The polyclonal antibody raised against Xenopus Glut1 specifically recognized this protein in an immunoblot of Xenopus retinal extract. (C) A section of Xenopus retina immunostained for Glut1 (green). Nuclei were counter-stained with Hoechst 33342 (blue). To the left is a schematic of a frog rod photoreceptor with an overlying RPE cell, illustrating the relative positions of individual subcellular compartments. Abbreviations are the same as in Fig. 1. Scale bar: 20 μm. (D–I) Xenopus retinal cryosections stained for Glut1 (green) and Na+/K+-ATPase (NKA, red; D–F) or phalloidin (red; G–I). Glut1 localized to the RPE and its microvilli, and to the rod photoreceptor plasma membrane in the synaptic, nuclear and inner segment layers. Labeling of the Na+/K+-ATPase (D–F) was used as a marker for the photoreceptor inner segment plasma membrane and calycal processes (arrowheads). Labeling of F-actin with phalloidin (G–I) was used as a marker for the RPE microvilli, in addition to the photoreceptor calycal processes. (J–L) Staining of two isolated rod photoreceptor fragments revealed Glut1 (green) on inner segment (IS) membranes and calycal processes (arrowheads) but not along the plasma membrane of outer segments (OS) marked by wheat germ agglutinin (WGA, red). Scale bars: 10 μm (D–L). Image published in: Gospe SM et al. (2010) Copyright © 2010. Image reproduced with permission of the Publisher, The Company of Biologists Ltd. Larger Image Printer Friendly View

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