Xenbase Image ID: 134456

Fig.3. Immunogold analysis of Glut1 in Xenopus retina sections. Longitudinal sections (A,B) and cross-sections (C,D) through Xenopus retina were processed for immunogold analysis. Gold particles (representatives marked by red arrowheads) were abundant on the fingerlike RPE microvilli (A) and their cross-sections (C). Rare, nonspecific particles (green arrowheads) were occasionally found within the rod outer segments, with no preference for the lateral plasma membrane; they were found at similar frequencies in control sections incubated with rabbit IgG control (B,D). (E) Gold particle labeling densities (particles per μm2 of membrane) were calculated for the outer segments and microvilli in both Glut1-stained and control sections. The mean labeling density for each type of structure in ten separate images is plotted with error bars representing 95% confidence intervals. MV, microvilli; OS, outer segment; ROS, rod outer segment. Scale bars: 200 nm. Image published in: Gospe SM et al. (2010) Copyright © 2010. Image reproduced with permission of the Publisher, The Company of Biologists Ltd. Larger Image Printer Friendly View

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