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Fig. 3 – Deletion of 15 or 40 C-terminal amino acids does not block cardiogenic activity of GATA4. (A) Expression of myh6 and odc1 in st. 34 animal caps expressing wt, 1–400, 1–425 and 1–425 mPKC (S419A; S420A) versions of rGATA4.Western blot analysis (lower panel) of protein levels at st. 10. (B) Nuclear localisation of 1–400, 1–425 and 1–425 rGATA4. (C) Embryos injected in anterior ectodermal blastomeres at 8–16 cell stage with 1–400, 1–425 and 1–425 were analysed for myl7 expression at st. 34. myl7 was detected in the heart (h) and in ectopic locations (arrows) of representative embryos (out of 40, 4–7 embryos showed ectopic cardiac expression). Image published in: Gallagher JM et al. (2014) Image downloaded from an Open Access article in PubMed Central. © 2014 The Authors. The Journal of Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society Larger Image Printer Friendly View

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