XB-IMG-134691
Xenbase Image ID: 134691
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Fig. 4 – Non-cardiogenic GATA4 mutants lacking N- or
C-terminus can be partially rescued by a heterologous
transcriptional activator. (A) 1–361 and 201–440 VP fusions
are stronger transcriptional activators than the wt rGATA4.
Embryos were injected at 1- or 2-cell stage with a firefly
luciferase reporter under the control of two GATA sites,
Renilla luciferase plasmid driven by the thymidine kinase
(TK) promoter and indicated mRNAs. Control-DNA alone
sample. Animal caps were excised at st. 8.5 and collected
for analysis 3 hours later. GATA-luc activity represents
relative fold activation (firefly luciferase activity normalised
by Renilla luciferase activity, with control (no mRNA)
sample set at 1). In three separate experiments 201–
440 + VP and 1–361 + VP were found to be considerably
more active than the wt rGATA4, with variable fold
differences but consistent trend of 201–440 + VP and
1–361 + VP showing 5–10 times greater activity than the wt
rGATA4. (B) VP16 minimal activation domain (VP) weakly
rescues cardiogenic activity of 1–361 and 201–440, as
assessed by expression of myl7 and myh6. (C) In contrast,
the ability of 1–361 and 201–440 VP fusions to induce the
expression of endothelial marker aplnr and a blood marker
hba3 is comparable to the wt rGATA4. Image published in: Gallagher JM et al. (2014) Image downloaded from an Open Access article in PubMed Central. © 2014 The Authors. The Journal of Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society Larger Image Printer Friendly View |