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Fig. 6 – Non-cell autonomous induction of cardiogenesis in animal cap explants by Sox17. Animal caps derived from cardiac actin–GFP (CA-GFP) embryos injected with 200 pg of Sox17 mRNA or uninjected (AC) were conjugated and cultured until st. 34. Different animal caps in each conjugate were distinguished by the presence of rhodamine-dextran in one of them. (A–D) Bright field images of representative examples of indicated types of explants. (A’–D’) Corresponding red and green fluorescence images (merged), showing contribution of rhodamine-dextran injected animal caps and the presence or absence of striated muscles (CA-GFP; Latinkic et al., 2003). CA-GFP activity was only detected in naïve, uninjected part of Sox17+AC conjugates, indicating non-cell autonomous cardiac induction. The number of conjugates showing the represented phenotype and the total number of conjugates are shown in the lower right corner (A–D). (E) Activity of Sox17 is shown by induction of endoderm marker a2m in st. 10 animal cap explants. (F) Inhibition of Sox17 function by Sox17::EnR does not block cardiogenesis by rGATA4. rGATA4 (400 pg/embryo) and Sox17::EnR (200 pg per embryo) were uniformly injected at 1- or 2-cell stage and RT-PCR was performed at st. 34 for myl7 and odc1 loading control. Image published in: Gallagher JM et al. (2014) Image downloaded from an Open Access article in PubMed Central. © 2014 The Authors. The Journal of Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society Larger Image Printer Friendly View

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