Xenbase Image ID: 134763

Figure 3. Smad6 antagonizes BMP signalling. (A) Overexpression of Smad6 in ventral cells induces the formation of a partial secondary axis (upper right, arrow) that contains immunoreactive muscle (middle right, arrow) while overexpression in dorsal cells does not induce patterning defects (upper and middle left). Overexpression of Smad6 in ectodermal cell explants (animal caps) causes formation of cement gland (lower right, arrows) while control explants maintain an epidermal appearance (lower left). (B) Smad6-injected, but not control ectodermal explants express cement gland-(XAG) and neural specific-(N-CAM, OtxA) genes but do not express the dorsal mesodermal marker α-actin (α-Act) as analysed by RT-PCR. EF-1α is an internal control. The faint Otx signal in the control explant lane was not observed in other experiments and is probably artefact. Image published in: Nakayama T et al. (1998) Copyright © 1998. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

Return to previous page