Xenbase Image ID: 134788

Fig. 1. Temporal expression patterns of XFrs3. (A) Schematic representation of the domain structure of XFrs3. Six putative tyrosine phosphorylation sites (Y191, 305, 350, 398, 445, and 480) are marked in red. Myr, myristoylation sequence (MGSCCS, amino acids 1-6); PTB, phosphotyrosine binding domain (aa 7-117); Grb2, putative Grb2-binding sites; Shp2, putative Shp2-binding sites. (B) Levels of XFrs3 transcript are analyzed by RT-PCR using RNA isolated from whole embryos at indicated stages. ODC is used as a loading control. (C, D) Spatial expression patterns of XFrs3 in mid-blastula (C, stage 9) and early gastrula (D, stage 10.5) are analyzed by RT-PCR. Embryos were dissected to animal, marginal and vegetal regions at stage 9, or bisected to dorsal and ventral regions at stage 10.5. WE indicates RNA from whole embryos, and WE (cut) indicates RNA isolated with all dissected regions combined. Levels of marker gene mRNA for each region are examined to access the accuracy of dissection: Sox3, animal region marker; Sox7 and VegT, vegetal region markers; Gsc, dorsal marker; Xvent1, ventral marker. For the negative control of RT-PCR (–RT), RNA from whole embryos was processed as others without reverse transcriptase. Image published in: Kim YJ et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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