Xenbase Image ID: 134793

Fig. 6. Knockdown of XFrs3 reduces the expression of Rx at tailbud stage. (A) XFrs3 MO or random negative control (Ran.) MO (7.5 pmole each/embryo) was injected into one dorsal blastomere of 4-cell embryos. Stage 30 embryos are analyzed by in situ hybridization for Rx. Embryos are viewed from the injected side. (B) XFrs3 MO or random MO (7.5 pmole each/embryo) was injected with or without XFrs3 mRNA (50 pg/embryo) into one dorsal blastomere of 4-cell embryos. Stage 20 embryos are analyzed by in situ hybridization for Pax6. Red and black arrows respectively indicate Pax6 expression in the eye on the MO alone and MO/mRNA-injected sides. For panel B, injections were carried out with 2 separate batches of embryos, and embryos were pooled and processed for in situ hybridization analyses. Image published in: Kim YJ et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue rax.L rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A X. laevis Throughout NF stage 29 and 30 retina optic vesicle pineal gland eye pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 20 lens retina optic vesicle cranial placode Image source: Published Larger Image Printer Friendly View

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