Xenbase Image ID: 134795

Fig. 8. XFrs3 is important for FGF or IGF-induced phosphorylation of ERK. Indicated combinations of XFrs3 or random MO (7.5 pmole each/embryo) along with wild type (WT) or mutant (4YF, 2YF, or 6YF) XFrs3 mRNA (50 pg each/embryo) were injected into animal region of 2-cell embryos. Animal caps were explanted at stage 8.5, treated with recombinant basic FGF (bFGF) (50 ng/mL) or IGFII (40 ng/mL) protein, and then harvested when sibling embryos reached stage 10. Activation of ERK (p-ERK) and Akt (p-Akt) are analyzed by immunoblotting. Actin is used as a loading control. For panel A, the total protein levels of ERK and Akt are also compared. For panel B, levels of XFrs3 proteins are compared by anti-Flag immunoblotting [Flag (XFrs3)]. (A) The numbers below p-ERK immunoblot (p-ERK/ERK) indicate relative intensities of p-ERK bands after normalization with corresponding ERK bands. The intensity of p-ERK band in MO non-injected and bFGF-treated sample is considered as 1. The intensities of p-ERK and ERK bands were measured by densitometry. (B) The numbers below p-ERK immunoblot (p-ERK/Actin) indicate relative intensities of p-ERK bands after normalization with corresponding Actin bands. The intensity of p-ERK band in MO/mRNA non-injected and bFGF-treated sample is considered as 1. Image published in: Kim YJ et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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