XB-IMG-134801
Xenbase Image ID: 134801
Supplement Fig. 6. Specificity of XFrs3 MO. 2-cell embryos were injected to equatorial region with indicated combinations of XFrs3-Flag mRNA with (+5' UTR) or without (-5' UTR) XFrs3 5' UTR (50 pg/embryo), and random or XFrs3 MO (7.5 pmole/embryo). Embryos were harvested at stage 10, and the expression levels of XFrs3-Flag are compared by anti-Flag immunoblotting [Flag (XFrs3)]. XFrs3 MO specifically inhibited the translation of XFrs3-Flag from mRNA containing the 5'UTR. Actin is used as a loading control. Image published in: Kim YJ et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |