XB-IMG-134811
Xenbase Image ID: 134811
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Fig. 3. Pax8 but not Pax2 loss of function inhibits tubule marker genes expression and pronephric tubule anlage formation at the early tailbud stage. (A) Whole mount in situ hybridization for tubule marker genes. Embryos were injected with MoPax8 (ae), MoPax2 (fj) or MoC (ko) at the 4-cell stage, in the equatorial region of both left blastomeres. They were cultured until tailbud stage 25, fixed and analyzed by in situ hybridization with the indicated probes. For each embryo, injected (ao) and uninjected (a, o) sides are shown. Pax8 but not Pax2 depletion strongly reduces expression of lhx1, hnf1b, pax2, hrt1, and evi1 in the pronephric area. (B) Immunofluorescence with an anti-laminin-1 antibody on transverse sections of stage 25 embryos injected with MoPax8 (p, q) or MoPax2 (r, s) on one side. For each embryo, injected (ps) and uninjected (ps) sides are shown. DAPI staining is shown in blue (q, q, s, s). On uninjected sides and MoPax2 injected side, the pronephric tubule anlage is visualized as a group of somatic cells radially organized, surrounded by laminin-1 immunostaining (p, q, r, s, r, s). No such cell arrangement is observed on the MoPax8 inejcted side. (pq). Laminin-1 is only detected in the extracellular matrix between the epidermis and the somatic mesoderm, as well as between the splanchnic mesoderm and the endoderm. Scale bar: 140 m. Image published in: Buisson I et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V.
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