Xenbase Image ID: 134812

Fig. 4. Inhibition of lhx1 expression in response to MoPax8 is rescued by expressing Pax8. Embryos were first injected at the 4-cell stage in both left blastomeres with MoPax8. They were split into two groups. Embryos from one group were injected again with RNA Pax8-GR. Dexamethasone was added to the culture medium at the end of gastrulation (stage 13). Phenotype was assessed by studying lhx1 expression by in situ hybridization at tailbud stage 25. Phenotypes were classified into three categories: strong inhibition (a), weak inhibition (b) and normal expression (c). Inhibition of pronephric lhx1 expression caused by MoPax8 was partially rescued by inducing Pax8 function just after completion of gastrulation. Histogram shows quantitative results from three independent experiments (d). n=number of analyzed embryos in total. Image published in: Buisson I et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue lhx1.L lim-1, Lim1, lim1, Xlim-1, Xlim1 X. laevis Throughout NF stage 25 pronephric mesenchyme dorsal lateral plate mesoderm spinal cord Image source: Published Larger Image Printer Friendly View

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