Xenbase Image ID: 134814

Fig. 6. Pax8 ectopic expression is sufficient to up-regulate hnf1b in ectoderm animal caps in absence of protein synthesis. (A and B) RT-qPCR analysis of hnf1b expression in blastula animal caps. (A) Embryos were injected with pax8 RNA in the animal pole of all four blastomeres at the 4-cell stage. Animal caps from injected and uninjected embryos were dissected at blastula stage 9, cultured for 3 h and processed for RT-qPCR. A significant increased of Hnf1b expression is observed in response to Pax8. Average values from five independent experiments. (B) Embryos were injected with pax8-GR RNA in the animal pole of all four blastomeres at the 4-cell stage. Animal caps from injected and uninjected embryos were dissected at blastula stage 9. They were pre-incubated with CHX for 30 min, or with 1MBS alone. CHX pre-incubated explants were then cultured for 3 h in CHX alone or in CHX+DEX; the others were cultured without CHX for 3 h in the presence or in absence of DEX. CHX alone causes an approximately fivefold increase of hnf1b expression. DEX treatment still causes a threefold increase of hnf1b expression relatively to CHX alone. Average values from three independent experiments. *P<0.05. Image published in: Buisson I et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V. Experiment + Assay Source Phenotypes and Disease Xla Wt + pax8 + CHX + DEX + animal cap amputation + NF9-10 (RT-PCR) Expression Phenotype increased amount hnf1b.L expression in animal cap Larger Image Printer Friendly View

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