Xenbase Image ID: 134817

Fig. 9. Pax8 regulates the expression of components of the wnt signaling pathway. (A) Expression of genes of the wnt signaling pathway analyzed by RT-qPCR in KF explants dissected at neurula stage 18 from uninjected or injected embryos with MoC or MoPax8. We considered the difference as significative (*) when the p Value between MoPax8 and MoC as well as between MoPax8 and uninjected was <0.05 when both -actine and ODC are used as endogenous reporter. dvl1 is downregulated and sfrp3 is upregulated in response to Pax8 depletion. In contrast, the expression of the secreted wnt inhibitors crescent, dkk1, dkk3, sfrp1, sfrp2, sfrp5, sizzled, of the wnt ligands wnt4, wnt5A, wnt8, wnt11b, wnt6, wnt9a, wnt9b as well as dvl2 and dvl3 is not modified. Average values from five independent experiments. (B) In situ hybridization with a dvl1 probe. Embryos were injected with MoPax8 or MoC at the 4-cell stage, in the equatorial region of both left blastomeres. At neurula stage 18, they were fixed, transversely bisected at the level of the pronephric area and analyzed by in situ hybridization. For each embryo, the dotted line delineates injected (left) and uninjected halves (right). Dvl1 is expressed in the intermediate mesoderm and its expression is inhibited in response to MoPax8 (arrow). (C, D) RT-qPCR analysis of dvl1 (C) and sfrp3 (D) expression in blastula animal caps. Embryos were injected with the indicated RNA (pax8, pax8-VP16, pax8O) in the animal pole of all four blastomeres at the 4-cell stage. Animal caps from injected and uninjected embryos were dissected at blastula stage 9, cultured for 3 h and processed for RT-qPCR. A significant increased of dvl1 expression is observed in response to Pax8-VP16. Sfrp3 is upregulated in response to Pax8O. Average values from four independent experiments. *P<0.05. Image published in: Buisson I et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue dvl1.L dishevelled1, dsh, dsh1, dvl-1, LOC108697641, Xdd1, Xdsh X. laevis Throughout NF stage 18 lateral plate mesoderm dorsal lateral plate mesoderm Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + pax8 MO + NF18 (in situ hybridization) Fig. 9B Expression Phenotype absent dvl1.L expression in dorsal lateral plate mesoderm absent dvl1.L expression in lateral plate mesoderm Larger Image Printer Friendly View

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