XB-IMG-134819
Xenbase Image ID: 134819
|
Supplementary Material. Fig. S1 MoPax8 and MoPax2 block endogenous embryonic Pax8 and Pax2 expression, respectively. (A) Immunofluorescence with anti-Pax8 (a-b) and anti-Pax2 (c, d) antibodies performed on transverse sections of embryos injected with MoPax8.2 (a, a), MoC (b, b) or MoPax2 (c, d) in both left blastomeres at the 4-cell. The KF region of injected (a, b) and uninjected side (b, b) is shown at the late neurula stage 18. The dorsal part of a stage 25 embryo containing the neural tube (NT) and the medial part containing the pronephros (arrow) are shown in c and d, respectively. The dotted line delineates injected (left) and the uninjected (right) halves. Pax8 is detected both in the splanchnic and somatic layers of the KF on the uninjected side, while it is absent on the MoPax8 injected side (a, a). MoC has no effect on the endogenous Pax8 expression (b). Pax2 expression in the neural tube and in the pronephros is inhibited on the MoPax2 injected side (c, d). Scale bar in a and b: 100 m, scale bar in c and d: 400 m (B) location of the Mo target sequences used in this study on pax2 and pax8 pseudoalleles. Nucleotides that differ between the two pseudoalleles are in blue. The start codon is in red. Image published in: Buisson I et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V.
Image source: Published Larger Image Printer Friendly View |