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Supplementary Material. Fig. S3 Pronephric phenotype induced by co-injection of MoPax8 and MoPax8.B. Whole mount in situ hybridization for tubule and glomus marker genes. Embryos were injected with MoPax8.B alone or in co-injection with MoPax8 at the 4-cell stage, in the equatorial region of both left blastomeres. They were cultured until the tailbud stages 3335 (A), early tailbud stage 2325 (B) and analyzed by in situ hybridization with specific probes for the indicated genes. For each embryo, injected (av) and uninjected (av) sides are shown. A strong reduction of the tubule marker genes (pax2, odf3, smp30, lhx1, hnf1b, pax2, hrt1, and evi1) expression is observed in response to the injection while the glomus markers wt1 and nephrin are still expressed. Insets show higher magnification of the anterior pronephric territory.
Image published in: Buisson I et al. (2015)
Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|
| pax2.L | LOC108697493, pax-2, pax2-a, pax2-b, XPax-2, XPax2 | X. laevis | Sometime during NF stage 33 and 34 to NF stage 35 and 36 | optic stalk
hindbrain
otic vesicle
midbrain-hindbrain boundary
spinal cord
pronephric kidney
pronephric nephrostome
pronephric duct
post-anal gut
pronephric tubule
proximal tubule
distal tubule
cement gland
| | cimap1a.L | odf3 | X. laevis | Sometime during NF stage 33 and 34 to NF stage 35 and 36 | pronephric kidney
pronephric nephrostome
| | rgn.L | regucalcin, smp30, xsmp-30, xsmp30 | X. laevis | Sometime during NF stage 33 and 34 to NF stage 35 and 36 | glomus
pronephric kidney
| | mecom.L | ecotropic viral integration site 1, evi-1, evi1, mecom-a, mecom-b, xEvi-1 | X. laevis | Sometime during NF stage 33 and 34 to NF stage 35 and 36 | pronephric tubule
proximal tubule
distal tubule
pronephric duct
hindbrain
midbrain
forebrain
cranial neural crest
| | nphs1.S | nephrin | X. laevis | Sometime during NF stage 33 and 34 to NF stage 35 and 36 | glomus
pronephric kidney
| | wt1.S | wt-1, wt1-a, wt1-b, Xwt-1, xwt1 | X. laevis | Sometime during NF stage 33 and 34 to NF stage 35 and 36 | glomus
pronephric kidney
primary heart field
| | lhx1.L | lim-1, Lim1, lim1, Xlim-1, Xlim1 | X. laevis | Throughout NF stage 25 | pronephric mesenchyme
lateral plate mesoderm
dorsal lateral plate mesoderm
| | hnf1b.L | hnf-1beta, hnf1-beta, hnf1beta, lfb3, tcf2, vhnf1 | X. laevis | Throughout NF stage 25 | glomus
pronephric mesenchyme
lateral plate mesoderm
dorsal lateral plate mesoderm
| | pax2.L | LOC108697493, pax-2, pax2-a, pax2-b, XPax-2, XPax2 | X. laevis | Throughout NF stage 25 | optic vesicle
otic vesicle
otic placode
midbrain-hindbrain boundary
spinal cord
post-anal gut
pronephric mesenchyme
lateral plate mesoderm
dorsal lateral plate mesoderm
| | hey1.S | bc8, chf2, herp2, hesr1, hrt-1, hrt1, oaf1, XHRT1 | X. laevis | Throughout NF stage 25 | glomus
pronephric mesenchyme
somite
head somite
trunk somite
presomitic mesoderm
rostral presomitic mesoderm
caudal presomitic mesoderm
forebrain
telencephalon
| | mecom.L | ecotropic viral integration site 1, evi-1, evi1, mecom-a, mecom-b, xEvi-1 | X. laevis | Throughout NF stage 25 | pronephric mesenchyme
hindbrain
midbrain
forebrain
lateral plate mesoderm
dorsal lateral plate mesoderm
neural crest
cranial neural crest
mandibular crest
hyoid crest
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