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Supplementary Material. Fig. S4. Hnf1b, but notosr2 and lhx1 expression, is inhibited in the neurula KF in response to the co-injection of MoPax8 and MoPax8.B. In situ hybridization for lhx1, osr2 and hnf1b genes. Embryos were injected with MoPax8.B alone or in co-injection with MoPax8 at the 4-cell stage, in the equatorial region of both left blastomeres. At neurula stage 18, they were fixed, transversely bisected at the level of the pronephric area and analyzed by in situ hybridization with the indicated probes. For each embryo, the dotted line indicates the frontier between the injected side (on the left) and the uninjected side (on the right). Hnf1b expression is inhibited in the pronephric area (arrow) on the injected side. Image published in: Buisson I et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue lhx1.L lim-1, Lim1, lim1, Xlim-1, Xlim1 X. laevis Throughout NF stage 18 pronephric mesenchyme lateral plate mesoderm dorsal lateral plate mesoderm osr2.S osr2-a, osr2-b, xosr2 X. laevis Throughout NF stage 18 pronephric mesenchyme lateral plate mesoderm dorsal lateral plate mesoderm hnf1b.L hnf-1beta, hnf1-beta, hnf1beta, lfb3, tcf2, vhnf1 X. laevis Throughout NF stage 18 lateral plate mesoderm dorsal lateral plate mesoderm Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + pax8 MO + NF18 (in situ hybridization) Sup Fig. S4 r1c1 Expression Phenotype decreased amount lhx1.L expression in lateral plate mesoderm Larger Image Printer Friendly View

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