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Supplementary Material. Fig. S5 Characterization of the kidney field explants. RT-qPCR analysis of lhx1, pax8, myf5, sox17beta and cytokeratin expression in KF explants and whole embryos. KF explants were dissected at neurula stage 18 from uninjected or injected embryos with MoC or MoPax8. The mRNAs renal markers Pax8 and lhx1 were enriched in the explants in contrast to the endodermal sox17beta and the somitic myf5 mRNAs. Explants including the fidney field and the overlying ectoderm, the epidermal gene cytokeratin is also expressed. Average values from four independent experiments. P<0.05, P<0.005, P<0.001. Image published in: Buisson I et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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