Xenbase Image ID: 134846

Fig. 2. Xdsh transcripts are present maternally and are equally distributed in different regions of the early blastula. Total RNA isolated from embryos at different developmental stages or from embryonic explants was analyzed by Northern blotting with specific antisense RNA probes. (A) Expression of Xdsh during embryogenesis: E, fertilized eggs; B, stage 7 blastulae; G, stage 11 gastrulae; N, stage 15 neurulae and T, tailbud embryos. (B) Spatial distribution of the Xdsh transcripts in stage 7 blastulae. Explants were isolated from A, animal; M, marginal; V, vegetal; D, dorsal; V¢, ventral regions; T, RNA prepared from whole embryos. Two embryo equivalents of total RNA were loaded per each lane. FN, Xwnt8 and Vg1-specific probes were used as controls. Fibronectin RNA (FN) is a control for loading. Xwnt8 transcripts are known to appear only after the midblastula transition (Christian et al., 1991). Vg1 RNA is a vegetally localized maternal mRNA (Rebagliati et al., 1985). Image published in: Sokol SY et al. (1995) Copyright © 1995. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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